Comparison of genetic diversity of farmed Oreochromis niloticus and wild unidentified tilapia (Wesafu) using microsatellite markers

Abstract. Ukenye EA, Megbowon I. 2023. Comparison of genetic diversity farmed Oreochromis niloticus and wild unidentified tilapia (Wesafu) using microsatellite markers. Biodiversitas 24: 2953-2957. Nile (Oreochromis Linnaeus, 1758) are cichlid species important nutritional economic value with good aquaculture potential. Assessing comparing the these will assist in information development for conservation management strategies to improve their overall productivity sustainable production. Using twenty fish specimens each from O. ecotype, Wesafu respectively, we examined between two species. Eight loci were utilized molecularly characterize compare farm based on variation levels. Genetic was investigated isolated, amplified resolved DNA obtained farm. The markers analysis revealed that exhibited higher than as evidenced by effective number alleles (1.756), Shannon index (0.596), observed expected heterozygosity values (0.682 0.400). All indices have declined populations due inbreeding. However, recorded more polymorphism (87%) (75%). Low differentiation found according fixation (-0.628) while Principal Coordinate Analysis (PCoA) demonstrated some level This finding provides insights into resource better minimize inbreeding aquaculture. We advise only broodstocks should be used restocking breeding program